Manufacturing Diagnostics 101

Wed 17 March, 16:00-17:30 GMT

Virtual symposium featuring talks on diagnostic devices, from research and development to implementation. Join breakout sessions with researchers from Africa, Asia, Latin America and beyond to continue discussions on quality assurance, device design, and more. Participate to share your experiences and join Reclone Network working groups.


The links to Parts 1&2 of the Symposium recordings and the notes from the breakout rooms can be found below! The recording links are within the Keynote sessions.
Time (GMT) Session Speaker
15:45 Virtual room opens
16:00 Opening Remarks Kayla
16:05 Developing In-house RPA and its Applications in CRISPR Diagnostics, specifically SARS-CoV-2 Dr Aimorn Homchan, Vidyasirimedhi Institute of Science and Technology, Thailand
Lightning Presentations
Multiple Speakers
FarmaCoop’s history as a recovered pharmaceutical laboratory and their challenges in manufacturing SARS-CoV-2 diagnostic tests
Bruno Di Mauro, as president of Farmacoop, and Biotechnologist Luciano Brum
17:05 Formal Closing Jenny
Breakout Rooms Open
Room 1: Sharing Best Practices for RPA Smitha Hedge
Room 2: What’s the best RT Adam Roberts
Room 3: Top Tips for Sharing Protocols Fernan Federici
Room 4: Show & Tell: Diagnostic Hardware Chinna Devarapu
17:30 Meeting Closes
Online room will be open for continued discussion


Dr. Aimorn Homchan, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

The collateral cleavage activity of CRISPR-associated nucleases, particularly Cas12 and Cas13, has been used as a basis for nucleic acid detection, including that of SARS-CoV-2. Common designs of CRISPR diagnostic methods combine an isothermal amplification step with a Cas-based detection step for maximal sensitivity and specificity. While CRISPR-based detection holds promise for use in low-resourced settings due to its accuracy, ease of use and minimal equipment requirement, the technology uses many biomolecular components, several of which are costly and proprietary. Here, I will describe our team’s efforts to locally produce and formulate recombinase-polymerase isothermal amplification (RPA), while concurrently improving several characteristics of RPA and CRISPR-based detection, particularly for the detection of SARS-CoV-2.

Bruno Di Mauro, President, and Luciano Brum, Biotechnologist, of Farmacoop

Farmacoop is a recovered company formed from Roux Ocefa Laboratory who went bankrupt after more than 80 years of work. The employees demanded to continue working and were able to create the cooperative. Through rebuilding the production and manufacturing plants, Farmacoop were able to receive certification to manufacture medical devices. Now, Farmacoop is developing a SARS-CoV-2 diagnostic test, named FarmaCov, using IgG antibodies to track positive cases as a complimentary assay to PCR that does not require highly skilled technicians. 


29 July 2020

View talks from the previous symposium on YouTube!
Speaker Link to Presentation
Daniel Ndima, CapeBio Harnessing African indigenous biological resources to develop “Best-in-Class” biologics for diagnostics
Dr Jacqueline Linnes, Purdue University, USA Designing user-friendly diagnostics

Reclone Symposium 1.0: Local Manufacturing of Reagents


10 June 2020

View talks from the previous symposium on YouTube!
Speaker Link to Presentation
Chiara Gandini, University of Cambridge, UK The Research in Diagnostics DNA Collection: an open source toolkit for production of enzymes for diagnostic research
Anibal Arce, Pontificia Universidad Catolica de Chile, Chile One-step RT-PCR from locally produced M-MLC + pfu-Sso7d
Alejandro Caro, AGROSAVIA, Colombia Optimization of a low-cost RNA extraction protocol for SARS-Cov2 based on magnetic beads
Ricardo Chavez, Scintia, Mexico ViroDet: a community effort on COVID-19 diagnostics for Mexico and Latin America
Cassi Henderson, University of Cambridge, UK Scaling up local manufacturing of reagents
Alejandro Nadra, University of Buenos Aires, Argentina Advances by ReClone Argentina
Thomas Graham, UC Berkeley, USA BEARMix - open-source master mix for 1-step RT-qPCR
Nadine Mowoh, MboaLab, Cameroon Quality Management Systems for enzyme production in Cameroon
Linda Kahl, SciScript and Beneficial Bio Biotechnology and the Public Domain: how to find out if technology is free and open