Reclone Reagents
Our DNA Collections
The Reclone Community steward a number of open source DNA collections for molecular biology tools and reagents
Get Our DNA
The Reclone Community steward a number of open source DNA collections for molecular biology tools and reagents
Get Protocols
Reclone Community members develop and share their protocols freely with the scientific community
Contribute!
Reclone Community members develop and share their plasmids and protocols freely with the scientific community
DNA Vectors
pTi Expression Vector
An open source vector for protein expression developed by Federici lab (PUC, Chile). Used in all our "ready to express" plasmids
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Developed by:
Fernan Federici, Lab de TecnologÃa Libre
pOpen v3
Open source cloning vector from Freegenes. Original DNA collections will ship in this. We recommend using pOpen v4.
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Developed by:
Keoni Gandall, Isaac Larkin, Free Genes, Endy Lab
pOpen v4
Open source cloning vector from Freegenes with inducible high-copy. Some recent DNA Collections will ship in this
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Developed by:
Keoni Gandall, Isaac Larkin, Free Genes, Endy Lab
DNA Collections
Legacy information about DNA collections can be found via the Free Genes website, Open Bioeconomy Lab spreadsheets and numerous other sources. The most up to date information is currently located at the Open DNA Collections github repository.
The Open DNA Collections Hub website is coming soon – watch this space!
Open Enzyme Collection (v1 & 2)
81 genes for "workhorse" enzymes for molecular biology, including DNA/RNA polymerases, ligases, reverse transcriptases.
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Developed by: Open Bioeconomy Lab, Lab de TecnologÃa Libre, Scott Pownall
Open Reporter Collection
32 commonly used reporter genes including chromoproteins, fluorescent proteins, luciferases and enzymes.
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Developed by: Open Bioeconomy Lab, Lab de TecnologÃa Libre, Scott Pownall
Research in Diagnostics Toolkit
16 "ready to express" plasmids for essential molecular diagnostics protocols. Expressed under T7 promoter and His-tagged.
- Developed by: Open Bioeconomy Lab, Lab de TecnologÃa Libre, Scott Pownall, Isaac Larkin, FreeGenes, Ginkgo Bioworks
E.coli Protein Expression Toolkit
A curated set of the parts you need to get started with recombinant protein expression in E. coli, in one handy toolkit.
- Developed by: Open Bioeconomy Lab, Lab de TecnologÃa Libre, Scott Pownall, Isaac Larkin, FreeGenes, Ginkgo Bioworks
Open Yeast Collection
92 DNA parts for engineering Saccharomyces cerevisiae and protein production in Pichia pastoris
- Developed by: Scott Pownall
B. subtilis Protein Secretion Toolkit
148 Bacillus subtilis SecTags for screening efficient protein secretion (provided in six arrays)
- Developed by: Friendzymes
Yeast Protein Expression Toolkit
Coming soon...
- Developed by: Scott Pownall
Your Ideas Here!
We are open to new suggestions and collaborations to make (more) useful collections. Suggest a new collection at the Reclone Forum!
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